Proteins involved in endocytic transit (Vacuolin pg2)

• Overview (pg.1) (pg.2)
• Coronin (pg.1)(pg.2)
• Actin
• Aip 1
• FcsA
• Lysozyme
• Vacuolin (pg.1)(pg.2)
• LvsB

Top: Overexpression of the vacuolin oligomerisation domain (brown dots) removes endogenous vacuolin (purple dots) from the endosomal membrane.

Right: Four cells expressing the oligmerization domain (green) at different levels. In the alternating image only the weakest cell (lower center) shows typical rings of endogenous vacuolin (red). In the other cells endogenous vacuolin becomes dispersed in the cytoplasm.

• Wienke, D., Drengk, A., Schmauch, C., Jenne, N. & Maniak, M.:
  Vacuolin, a flotillin/reggie-related protein from Dictyostelium oligomerizes for endosome association. Eur. J. Cell Biol., 85, 991-1000, 2006

Function: Oligomerisation provides stronger target affinity to otherwise weakly binding PHB domains such as in vacuolin or in mammalian flotillin.

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