Proteins involved in endocytic transit (Vacuolin pg1)
Vacuolin pg1 text
Top: Vacuolin (purple dots), a protein related to flotillin/reggie from vertebrates is a marker for late, neutral endosomes.
Right: Fluid-phase-marker (red) reaches the GFP-vacuolin decorated endosome after one hour.
Vacuolin pg1 Text 2
Function: Cells lacking the gene encoding vacuolin B have a single giant neutral endosome and a prolonged transit of endocytic cargo. Vacuolin A mutants are phenotypically normal. Vacuolin has also been used to target cytoskeletal proteins to the late endosome to analyse the function of the actin coat (click here for more).
References:
Rauchenberger et al., 1997
- Rauchenberger, R., Hacker, U., Murphy, J., Niewöhner, J. & Maniak, M.: Coronin and vacuolin identify consecutive stages of a late, actin-coated endocytic compartment in Dictyostelium. Current Biology 7, 215-218, 1997
Jenne et al., 1998
- Jenne, N., Rauchenberger, R., Hacker, U., Kast, T. & Maniak, M.: Targeted gene disruption reveals a role for vacuolin B in the late endocytic pathway and exocytosis. J. Cell Sci. 111, 61-70, 1998.