Proteins involved in lipid metabolism (FcsB) pg. 1

• Overview (pg.1) (pg.2)
• FcsA
• Plin (pg.1)(pg.2)
• Smt1, Ldp, Net4
• Dgat1
• Dgat2
• FcsB (pg.1)(pg.2)

Top: The FcsB Protein (red dots) is translated into the endoplasmic reticulum and then further transported to peroxisomes, which is a very rare and unusual pathway.

Right: Insertion of a myc epitope into the middle of the protein (red) allows the passage through the entire pathway, yielding a peroxisomal pattern. In contrast, adding the GFP-tag to the C-terminus of FcsB (green) blocks its egression from the ER.

Function: FcsB activates fatty acids (FA) by addition of coezyme A (CoA) in the lumen of the peroxisome for subsequent degradation via ß-oxidation.

Reference:

• Paschke, P., Pawolleck, N., Haenel, F., Otto, H., Rühling, H., Maniak, M.: The isoform B of the Dictyostelium long-chain fatty-acyl-coenzyme A synthetase is initially inserted into the ER and subsequently provides peroxisomes with an activity important for efficient phagocytosis. Eur. J. Cell Biol. 91, 717-727, 2012