Proteins involved in lipid metabolism (Plin pg. 2)

• Overview (pg.1) (pg.2)
• FcsA
• Plin (pg.1)(pg.2)
• Smt1, Ldp, Net4
• Dgat1
• Dgat2
• FcsB (pg.1)(pg.2)

Top: In a Dictyostelium cell infected with the intracellular pathogen Mycobacterium marinum, fat (yellow) is transferred from cellular lipid droplets to the invader (blue). Surprisingly, the surface of Mycobacteria is then coated with the perilipin protein of the host (brown dots).

Right: Overview and magnification of an infected Dictyostelium cell showing GFP- Plin (green) surrounding Mycobacteria expressing a red fluorescent protein (red).

Function: In a perilipin knockout strain, the growth of Mycobacteria in the host cytoplasm is reduced, indicating that the perilipin coat is protective against host defence mechanisms.

Reference:

• Barisch, C., Paschke, P., Hagedorn, M., Maniak, M., Soldati, T.: Lipid droplet dynamics at early stage of Mycobacterium marinum infection in Dictyostelium. Cell. Microbiol. 17, 1332-1349 , 2015