Proteins involved in lipid metabolism (FcsB) pg. 2

• Overview (pg.1) (pg.2)
• FcsA
• Plin (pg.1)(pg.2)
• Smt1, Ldp, Net4
• Dgat1
• Dgat2
• FcsB (pg.1)(pg.2)

Top: The endosomal enzyme FcsA (green dots) can be forced to enter peroxisomes solely by the addition of a three amino acid signal (Ser-Lys-Leu) to its C-terminal end.

Right: GFP-FcsA-SKL (green) enters peroxisomes (red).

Function: FcsA artificially targeted to peroxisomes can cure the phagocytic defect seen in a FcsB mutant, proving that the peroxisomal acyl-CoA synthetase activity is required for this process.

Reference:

• Paschke, P., Pawolleck, N., Haenel, F., Otto, H., Rühling, H., Maniak, M.: The isoform B of the Dictyostelium long-chain fatty-acyl-coenzyme A synthetase is initially inserted into the ER and subsequently provides peroxisomes with an activity important for efficient phagocytosis. Eur. J. Cell Biol. 91, 717-727, 2012.